Journal: Cancers

Article Title: Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas

doi: 10.3390/cancers13205113

Figure Lengend Snippet: Hypermethylation and histone modifications account for transcriptional downregulation of DIRAS-1 and DIRAS-2. ( A ) Increase in DIRAS-1 and DIRAS-2 mRNA expression in U251MG and Hs683 cells after treatment with 5-azacytidine, ( B ) after treatment with trichostatin A (TSA), and ( C ) after combinational treatment with 5-azacytidine and trichostatin A. ( D ) Increase in DIRAS-1 and DIRAS-2 promoter DNA bound to acetylated H3, indicating DIRAS-1 and DIRAS-2 promoter euchromatinization after TSA treatment in U251MG and Hs683 cells (n.s.: not significant, * p ≤ 0.05, ** p ≤ 0.002, *** p ≤ 0.001). Original figure in .

Article Snippet: U251MG and Hs683 glioblastoma cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Expressing

Journal: Cancers

Article Title: Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas

doi: 10.3390/cancers13205113

Figure Lengend Snippet: Functional analyses of the role of DIRAS-1 and DIRAS-2 in human gliomas. ( A ) Western blot analysis showing DIRAS-1 and DIRAS-2 overexpression in U251MG and Hs683 cells. Detailed information about Western Blots can be found in the . ( B ) Cell proliferation after overexpression of DIRAS-1 or DIRAS-2 and ( C ) chemosensitivity to lomustin after overexpression of DIRAS-1 or DIRAS-2 in U251MG and Hs683 cells (n.s.: not significant, * p ≤ 0.05, ** p ≤ 0.002). Original figure in .

Article Snippet: U251MG and Hs683 glioblastoma cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Functional Assay, Western Blot, Over Expression

Journal: Molecular Oncology

Article Title: Hyaluronic acids mediate the infiltration, migration, and M2 polarization of macrophages: evaluating metabolic molecular phenotypes in gliomas

doi: 10.1002/1878-0261.13315

Figure Lengend Snippet: HA in the infiltration, migration, and polarization of macrophages. (A) Multiplex immunofluorescence staining of CD68 (pink), CD163 (red), and DAPI (blue) on glioma whole tissue section (10× and 40×). Scale bars: 400 and 80 μm, respectively. (B) Transwell assay for migration of HMC3 in the control group and hyaluronidase group after co‐culture of HMC3 and glioma cell lines, HS683, U251, and U87. Scale bar: 100 μm. (C) Flow diagram of the co‐culture system for Transwell assay. (D) Statistical analysis of Transwell assay for migration of HMC3 after co‐culture of HMC3 and glioma cell lines, HS683, U251, and U87. Statistical analysis was performed using an unpaired Student's t ‐test. (E) Flow diagram of the co‐culture system for immunofluorescence staining. (F) Immunofluorescence staining of CD68 and CD163 in the control and hyaluronidase groups after co‐culture of HMC3 and HS683. Scale bar: 25 μm. (G). Immunofluorescence staining of CD68 and CD11c in the control and hyaluronidase groups after co‐culture of HMC3 and HS683. Scale bar: 25 μm. (H). Statistical analysis of immunofluorescence staining of CD68 and CD163. Statistical analysis was performed using an unpaired Student's t ‐test. (I). Statistical analysis of immunofluorescence staining of CD68 and CD11c. Statistical analysis was performed using an unpaired Student's t ‐test. Data are represented as mean ± SD. The results shown are representative of three independent experiments. NS, not statistically significant; ** P < 0.01; *** P < 0.001.

Article Snippet: HS683, U251, U87, and HMC3 cell lines were purchased from iCell ( http://www.icellbioscience.com ).

Techniques: Migration, Multiplex Assay, Immunofluorescence, Staining, Transwell Assay, Control, Co-Culture Assay

Journal: Cancer Management and Research

Article Title: The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy

doi: 10.2147/CMAR.S463694

Figure Lengend Snippet: EMILIN3 gene function experiment in LN229. qRT-PCR transfected LN229 human glioma cells efficiently (A) . The CCK-8 experiment and live/dead staining findings demonstrated that overexpression of the EMILIN3 gene may increase LN229 cell line proliferation and vitality ( B and C ). Overexpression of the EMILIN3 gene enhances the migration, invasiveness, and colony formation of the LN229 cell line (D-F ).

Article Snippet: The LN229 and HS-683 human glioma cell line were purchased from iCell Bioscience Inc, and grown in DMEM media (Gibco, USA) with 10% foetal bovine serum (BI, USA) and 1g/mL penicillin/streptomycin (Hyclone, USA) at 37 degrees Celsius and 5% carbon dioxide.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Over Expression, Migration